核黃素激酶

跳轉到: 導航, 搜索
riboflavin kinase
核黃素激酶
Riboflavkinase.png
Thermoplasma acidophilum中的核黃素激酶晶體結構[1]
識別碼
EC編號 2.7.1.26
CAS號 9032-82-0
資料庫
IntEnz IntEnz瀏覽
BRENDA BRENDA入口
ExPASy NiceZyme瀏覽
KEGG KEGG入口
MetaCyc 代謝路徑
PRIAM 概述
PDB RCSB PDB PDBe PDBsum
基因本體 AmiGO / EGO
Riboflavin kinase
核黃素激酶
PDB 1s4m EBI.jpg
crystal structure of flavin binding to fad synthetase from thermotoga maritina
鑒定
標誌 Flavokinase
Pfam(蛋白家族查詢站) PF01687
InterPro(蛋白數據整合站) IPR015865
SCOP(蛋白結構分類數據站) 1mrz
Riboflavin kinase
核黃素激酶
鑒定
標誌 Riboflavin_kinase
Pfam(蛋白家族查詢站) PF01687
InterPro(蛋白數據整合站) IPR015865

核黃素激酶英語:riboflavin kinaseEC 2.7.1.26)是一個催化以下化學反應的酶:

ATP + 核黃素 \rightleftharpoons ADP + FMN

酶催化的反應的底物ATP核黃素產物是ADP和黃素單核苷酸(FMN)。

但是,在古菌核黃素激酶EC 2.7.1.161)中,常使用CTP而非ATP作為反應底物,催化如下反應:

CTP + 核黃素 \rightleftharpoons CDP + FMN [2]

核黃素激酶也在許多細菌中發現,具有類似的功能,但存在若干數量的胺基酸不同。

目錄

反應

核黃素 + XTP → FMN + XDP

Riboflavin is converted into catalytically active cofactors (FAD and FMN) by the actions of riboflavin kinase (EC 2.7.1.26), which converts it into FMN, and FAD synthetase (EC 2.7.7.2), which adenylates FMN to FAD. Eukaryotes usually have two separate enzymes, while most prokaryotes have a single bifunctional protein that can carry out both catalyses, although exceptions occur in both cases. While eukaryotic monofunctional riboflavin kinase is orthologous to the bifunctional prokaryotic enzyme,[3] the monofunctional FAD synthetase differs from its prokaryotic counterpart, and is instead related to the PAPS-reductase family.[4] The bacterial FAD synthetase that is part of the bifunctional enzyme has remote similarity to nucleotidyl transferases and, hence, it may be involved in the adenylylation reaction of FAD synthetases.[5]

This enzyme belongs to the family of transferases, to be specific, those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is ATP:riboflavin 5'-phosphotransferase. This enzyme is also called flavokinase. This enzyme participates in riboflavin metabolism.

結構

截止2007年底,這類酶中已有14個三級結構被解決,在PDB的登陸代碼為1N051N061N071N081NB01NB91P4M1Q9S2P3M2VBS2VBT3CTA2VBU2VBV

參考文獻

  1. PDB 3CTA; Bonanno, J.B., Rutter, M., Bain, K.T., Mendoza, M., Romero, R., Smith, D., Wasserman, S., Sauder, J.M., Burley, S.K., Almo, S.C.. Crystal structure of riboflavin kinase from Thermoplasma acidophilum. 2008. 
  2. Ammelburg M, Hartmann MD, Djuranovic S, Alva V, Koretke KK, Martin J, Sauer G, Truffault V, Zeth K, Lupas AN, Coles M. A CTP-Dependent Archaeal Riboflavin Kinase Forms a Bridge in the Evolution of Cradle-Loop Barrels. Structure.. 2007, 12 (12): 1577–90. doi:10.1016/j.str.2007.09.027. PMID 18073108. 
  3. Osterman AL, Zhang H, Zhou Q, Karthikeyan S. Ligand binding-induced conformational changes in riboflavin kinase: structural basis for the ordered mechanism. Biochemistry. 2003, 42 (43): 12532–8. doi:10.1021/bi035450t. PMID 14580199. 
  4. Galluccio M, Brizio C, Torchetti EM, Ferranti P, Gianazza E, Indiveri C, Barile M. Over-expression in Escherichia coli, purification and characterization of isoform 2 of human FAD synthetase. Protein Expr. Purif.. 2007, 52 (1): 175–81. doi:10.1016/j.pep.2006.09.002. PMID 17049878. 
  5. Srinivasan N, Krupa A, Sandhya K, Jonnalagadda S. A conserved domain in prokaryotic bifunctional FAD synthetases can potentially catalyze nucleotide transfer. Trends Biochem. Sci.. 2003, 28 (1): 9–12. doi:10.1016/S0968-0004(02)00009-9. PMID 12517446. 

延伸閱讀

  • CHASSY BM, ARSENIS C, MCCORMICK DB. THE EFFECT OF THE LENGTH OF THE SIDE CHAIN OF FLAVINS ON REACTIVITY WITH FLAVOKINASE. J. Biol. Chem.. 1965, 240: 1338–40. PMID 14284745. 
  • GIRI KV, KRISHNASWAMY PR, RAO NA. [http//www.ncbi.nlm.nih.gov/pmc/articles/PMC1196627/ Studies on plant flavokinase]. Biochem. J.. 1958, 70 (1): 66–71. PMID 13584303. PMC 1196627. 
  • KEARNEY EB. The interaction of yeast flavokinase with riboflavin analogues. J. Biol. Chem.. 1952, 194 (2): 747–54. PMID 14927668. 
  • McCormick DB and Butler RC. Substrate specificity of liver flavokinase. Biochim. Biophys. Acta. 1962, 65 (2): 326–332. doi:10.1016/0006-3002(62)91051-X. 
  • Sandoval FJ, Roje S. An FMN hydrolase is fused to a riboflavin kinase homolog in plants. J. Biol. Chem.. 2005, 280 (46): 38337–45. doi:10.1074/jbc.M500350200. PMID 16183635. 
  • Solovieva IM, Tarasov KV, Perumov DA. Main physicochemical features of monofunctional flavokinase from Bacillus subtilis. B. Mosc, Biochemistry. (2): 177–81. PMID 12693963. 
  • Solovieva IM, Kreneva RA, Leak DJ, Perumov DA. The ribR gene encodes a monofunctional riboflavin kinase which is involved in regulation of the Bacillus subtilis riboflavin operon. Microbiology.. Pt 1, 145: 67–73. doi:10.1099/13500872-145-1-67. PMID 10206712. 

此條目包含有源於Pfam以及InterPro的屬於公有領域的文本 IPR015865

參考來源

關於「核黃素激酶」的留言: Feed-icon.png 訂閱討論RSS

目前暫無留言

添加留言

更多醫學百科條目

個人工具
名字空間
動作
導航
功能菜單
工具箱